Composite

Part:BBa_K2997008:Design

Designed by: Ting-Han Sung   Group: iGEM19_NCKU_Tainan   (2019-10-12)


Native promoter-pchR-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 1769
    Illegal SpeI site found at 3174
    Illegal PstI site found at 3188
    Illegal NotI site found at 7
    Illegal NotI site found at 3181
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3174
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 3174
    Illegal PstI site found at 3188
    Illegal NgoMIV site found at 1227
    Illegal AgeI site found at 210
    Illegal AgeI site found at 1110
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1136
    Illegal BsaI.rc site found at 3096


Design Notes

BBa_K2997008


Source

BBa_K2997008

References